Comparison of a Beta-propiolactone Inactivated Four-strain With Three Single-strain Newcastle Disease Vaccines

MACK and Chotisen (1955) demonstrated that chickens vaccinated with a beta-propiolactone (BPL)-inactivated Newcastle disease virus (NDV) vaccine were 100% resistant when challenged 16 days after vaccination. However, no attempt was made to determine which NDV strains were the best immunogenic agent. The Mack and Chotisen report did, however, stimulate much interest in the development and use of BPL-inactivated NDV vaccines. It is quite possible that BPL-inactivated vaccines will prove to be best suited for the eradication of this disease. Appleton et al. (1963) compared the efficacy of BPL and formalin as inactivating agents of the Roakin strain of NDV and concluded that formalin was better. In this 1963 report, they discussed variables relating to NDV inactivation. Grun and Hudson (1966) compared a one-strain, Gilbert-Boney (GB), whole embryo BPL-inactivated NDV vaccine with a vaccine prepared from AA fluid containing four strains (LaS, Ho, IPa, GB). Six BPL-inactivated NDV vaccines which contained…