MHC I级
抗原
细胞毒性T细胞
CD8型
癌症免疫疗法
卵清蛋白
免疫疗法
主要组织相容性复合体
癌症疫苗
免疫系统
交叉展示
材料科学
免疫学
生物
生物化学
体外
作者
Fengqiang Cao,Ming Yan,Yijia Liu,Lanxia Liu,Guilei Ma
标识
DOI:10.1002/adhm.201701439
摘要
Abstract Cancer vaccines aim to induce a strong major histocompatibility complex class I (MHC‐I)‐restricted CD8 + cytotoxic T‐cell response, which is an important prerequisite for successful cancer immunotherapy. Herein, a hyaluronic acid (HA) and antigen (ovalbumin, OVA)‐decorated gold nanoparticle (AuNPs)‐based (HA‐OVA‐AuNPs) vaccine is developed for photothermally controlled cytosolic antigen delivery using near‐infrared (NIR) irradiation and is found to induce antigen‐specific CD8 + T‐cell responses. Chemical binding of thiolated HA and OVA to AuNPs facilitates antigen uptake of dendritic cells via receptor‐mediated endocytosis. HA‐OVA‐AuNPs exhibit enhanced NIR absorption and thermal energy translation. Cytosolic antigen delivery is then permitted through the photothermally controlled process of local heat‐mediated endo/lysosome disruption by laser irradiation along with reactive oxygen species generation, which helps to augment proteasome activity and downstream MHC I antigen presentation. Consequently, the HA‐OVA‐AuNPs nanovaccine can effectively evoke a potent anticancer immune response in mice under laser irradiation. This NIR‐responsive nanovaccine is promising as a potent vaccination method for improving cancer vaccine efficacy.
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