Novel series os pea symbiotic mutants induced in the SGE line

Using ethylmethansulphonate the chemical mutagenesis of the pea laboratory line SGE was performed. During analysis of 425 families (2069 plants) of Мsub2/sub progeny 45 putative mutants were selected, among them 30 mutants forming ineffective nodules (Fixsup–/sup phenotype), 13 mutantsunable to form nodules (Nodsup–/sup phenotype), and 2 mutants forming a few nodules (Nodsup+/–/sup phenotype). For 1 Nodsup–/sup and 5 Fixsup–/sup mutants monogenic inheritance and recessive phenotype manifestation were demonstrated. For Fixsup– /supmutant SGEFixsup–/sup–9 an additional mutation leading to Nodsup+/–/sup phenotype was shown. Complementation analysis showed that the mutant phenotype of the SGEFixspan style="font-size:11px"sup-/sup - /span5 line is caused by a mutation in the sym33 gene, of theSGEFixsup–/sup–6 linein the sym40 gene, of the SGEFixsup–/sup–7 line in the sym27 gene, and of the SGEFixsup–/sup–8 linein the sym25 gene.