Fluorescent TPA@GQDs Probe for Sensitive Assay and Quantitative Imaging of Hydroxyl Radicals in Living Cells

A fluorescent probe TPA@GQDs is fabricated by the conjugation of terephthalic acid (TPA) on the surface of graphene quantum dots (GQDs). The TPA@GQDs probe not only has favorable dispersibility but also exhibits excellent fluorescence stability over a wide pH range and high ionic strength and favorable antiphotobleaching ability. The great fluorescence enhancement of TPA@GQDs induced by the reaction between TPA and hydroxyl radicals makes the TPA@GQDs a powerful probe for the sensitive assay of hydroxyl radicals, giving rise to a low detection limit down to 12 nmol L–1. Meanwhile, the obtained fluorescent TPA@GQDs probe shows low cytotoxicity and favorable biocompatibility. Its potential in bioimaging is demonstrated by the quantitative fluorescent imaging of hydroxyl radicals in living HeLa cells under different circumstances, which enables the opportunities to study hydroxyl radicals dynamics in living cells.