自愈水凝胶
核糖核酸
细胞内
胞浆
生物物理学
聚合物
化学
蛋白质生物合成
纳米技术
材料科学
细胞生物学
生物化学
生物
基因
高分子化学
酶
有机化学
作者
Hideki Nakamura,Albert A. Lee,Ali Afshar,Shigeki Watanabe,Elmer Rho,Shiva Razavi,Allister Suarez,Yu Lin,Makoto Tanigawa,Brian Huang,Robert DeRose,Diana Bobb,William Hong,Sandra B. Gabelli,John Goutsias,Takanari Inoue
出处
期刊:Nature Materials
[Springer Nature]
日期:2017-11-06
卷期号:17 (1): 79-89
被引量:109
摘要
Some protein components of intracellular non-membrane-bound entities, such as RNA granules, are known to form hydrogels in vitro. The physico-chemical properties and functional role of these intracellular hydrogels are difficult to study, primarily due to technical challenges in probing these materials in situ. Here, we present iPOLYMER, a strategy for a rapid induction of protein-based hydrogels inside living cells that explores the chemically inducible dimerization paradigm. Biochemical and biophysical characterizations aided by computational modelling show that the polymer network formed in the cytosol resembles a physiological hydrogel-like entity that acts as a size-dependent molecular sieve. We functionalize these polymers with RNA-binding motifs that sequester polyadenine-containing nucleotides to synthetically mimic RNA granules. These results show that iPOLYMER can be used to synthetically reconstitute the nucleation of biologically functional entities, including RNA granules in intact cells. An approach to form protein-based hydrogels in living cells that resemble physiological hydrogel-like size-dependent molecular sieves is presented. Synthetic RNA granules mimics are obtained by functionalizing these entities with RNA-binding motifs.
科研通智能强力驱动
Strongly Powered by AbleSci AI