末端脱氧核苷酸转移酶
基因
生物
生殖系
剧目
分子生物学
遗传学
T细胞受体
重组激活基因
受体
基因重排
重组信号序列
重组
T细胞
免疫系统
细胞凋亡
物理
声学
标记法
作者
Toshihisa Komori,Ami Okada,Valerie Stewart,Frederick W. Alt
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1993-08-27
卷期号:261 (5125): 1171-1175
被引量:433
标识
DOI:10.1126/science.8356451
摘要
During the assembly of immunoglobulin and T cell receptor variable region genes from variable (V), diversity (D), and joining (J) segments, the germline-encoded repertoire is further diversified by processes that include the template-independent addition of nucleotides (N regions) at gene segment junctions. Terminal deoxynucleotidyl transferase (TdT)-deficient lymphocytes had no N regions in their variable region genes, which shows that TdT is responsible for N region addition. In addition, certain variable region genes appeared at increased frequency in TdT-deficient thymocytes, which indicates that N region addition also influences repertoire development by alleviating sequence-specific constraints imposed on the joining of particular V, D, and J segments.
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