In this paper, an AlGaN/GaN based MIS-HEMT using Al2O3 dielectric as gate insulator was fabricated. We adopted the patterned sapphire as the substrate (PSS) of high-quality AlGaN GaN epitaxial layers. We also studied the influence of different gate-drain space (Lgd) on breakdown voltage (VBD) and on-state resistance (Ron) of GaN HEMT fabricated on patterned sapphire substrate. A breakdown voltage of 105 V was obtained with the Lgdof 2 μm and gate width of 32 μm. The specific on resistance was 4.7 Ω·mm when Lgdequals 0.5 μm. Meanwhile, it is found that the devices fabricated on the wet-etched pyramidal patterned sapphire substrate GaN exhibit better drive current stability than that of the devices on the conventional GaN-on-Sapphire substrate.