维甲酸
生物
畸胎瘤
胚胎癌
P19电池
细胞分化
干细胞
细胞生物学
细胞培养
维甲酸受体
维甲酸
分子生物学
生物化学
成体干细胞
遗传学
基因
作者
Sidney Strickland,Vijak Mahdavi
出处
期刊:Cell
[Elsevier]
日期:1978-10-01
卷期号:15 (2): 393-403
被引量:1430
标识
DOI:10.1016/0092-8674(78)90008-9
摘要
Embryonal carcinoma cells, the stem cells of teratocarcinomas, usually undergo extensive differentiation in vivo and in vitro to a wide variety of cell types. There exist, however, several embryonal carcinoma cell lines that have almost completely lost the capacity to differentiate, so that the cells are propagated primarily as the stem cells. Using one such cell line, F9, we have found that retinoic acid at concentrations as low as 10(-9) M induces multiple phenotypic changes in the cultures in vitro. These changes include morphological alteration at the resolution of the light microscope, elevated levels of plasminogen activator production, sensitivity to cyclic AMP compounds and increased synthesis of collagen-like proteins. The nature of these changes, as well as their independence of the continued presence of retinoic acid, are consistent with the proposition that retinoic acid induces differentiation of embryonal carcinoma cells into endoderm.
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