DISTRIBUTION OF P2X RECEPTORS IN THE URINARY BLADDER AND THE URETER OF THE RAT

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Jun 2000DISTRIBUTION OF P2X RECEPTORS IN THE URINARY BLADDER AND THE URETER OF THE RAT HYE YEON LEE, MICHELLE BARDINI, and GEOFFREY BURNSTOCK HYE YEON LEEHYE YEON LEE , MICHELLE BARDINIMICHELLE BARDINI , and GEOFFREY BURNSTOCKGEOFFREY BURNSTOCK View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)67618-5AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: One means of assessing the presence of purinoceptors and their possible participation in signaling events in tissues is through use of specific antibodies and immunohistochemical methods. A thorough immunohistochemical screening for the presence of P2X receptors on bladder and ureter sections has been performed. Materials and Methods: Distribution of P2X receptor subtypes in rat bladder and ureter has been investigated using specific polyclonal antibodies to P2X1 through to P2X7 receptor subtypes with immunohistochemical methods. Results: In both the bladder detrusor muscle and the ureteral muscle (as well as the accompanying arteries) P2X1 immunoreactivity was associated with the smooth muscle membranes. Non-membrane associated smooth muscle reactivity was seen with P2X2> P2X5 = P2X6. P2X3 immunoreactivity was seen within nerve bundles in detrusor muscle only. The fine capillary network supplying bladder and ureter smooth muscle and lamina propria was visualized with P2X4 immunoreactivity, membranes of urothelial cells gave a strong reaction with P2X5, whereas P2X6 immunostained the thin basement membrane beneath the urothelium. Nuclear staining was seen with P2X7 in the urothelium but more prominent in the bladder than in the ureter. 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Google Scholar From the Autonomic Neuroscience Institute, Royal Free and University College Medical School, Rowland Hill Street, London, United Kingdom© 2000 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited BySUI G, WU C and FRY C (2018) Electrical Characteristics of Suburothelial Cells Isolated From the Human BladderJournal of Urology, VOL. 171, NO. 2, (938-943), Online publication date: 1-Feb-2004.IGAWA Y, ZHANG X, NISHIZAWA O, UMEDA M, IWATA A, TAKETO M, MANABE T, MATSUI M and ANDERSSON K (2018) CYSTOMETRIC FINDINGS IN MICE LACKING MUSCARINIC M2 OR M3 RECEPTORSJournal of Urology, VOL. 172, NO. 6 Part 1, (2460-2464), Online publication date: 1-Dec-2004.Pandita R and Andersson K (2018) Intravesical Adenosine Triphosphate Stimulates the Micturition Reflex in Awake, Freely Moving RatsJournal of Urology, VOL. 168, NO. 3, (1230-1234), Online publication date: 1-Sep-2002.YOSHIMURA N and CHANCELLOR M (2018) Current and Future Pharmacological Treatment for Overactive BladderJournal of Urology, VOL. 168, NO. 5, (1897-1913), Online publication date: 1-Nov-2002.O'REILLY B, KOSAKA A, KNIGHT G, CHANG T, FORD A, RYMER J, POPERT R, BURNSTOCK G and McMAHON S (2018) P2X RECEPTORS AND THEIR ROLE IN FEMALE IDIOPATHIC DETRUSOR INSTABILITYJournal of Urology, VOL. 167, NO. 1, (157-164), Online publication date: 1-Jan-2002. Volume 163Issue 6June 2000Page: 2002-2007 Advertisement Copyright & Permissions© 2000 by American Urological Association, Inc.KeywordsP2X receptorsbladderureterratMetricsAuthor Information HYE YEON LEE More articles by this author MICHELLE BARDINI More articles by this author GEOFFREY BURNSTOCK More articles by this author Expand All Advertisement PDF DownloadLoading ...