亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Different effects of anti-VEGF drugs (Ranibizumab, Aflibercept, Conbercept) on autophagy and its effect on neovascularization in RF/6A cells

自噬 脉络膜新生血管 阿柏西普 药理学 新生血管 血管抑制剂 血管内皮生长因子受体 细胞凋亡 血管生成 免疫印迹 血管内皮生长因子 标记法 黄斑变性 化学 内皮干细胞 流式细胞术 贝伐单抗 癌症研究 细胞生物学 体外 生物 医学 分子生物学 眼科 生物化学 内科学 化疗 基因
作者
Yì Wáng,Yang Yao,Hongsong Li,Bing-Hui Wu,Huiqin Lu,Jing Cheng,Zhe Liu,Junhui Du
出处
期刊:Microvascular Research [Elsevier BV]
卷期号:138: 104207-104207 被引量:10
标识
DOI:10.1016/j.mvr.2021.104207
摘要

Abstract Introduction Choroidal neovascularization (CNV) is the main pathological change of wet age-related macular degeneration. Anti-VEGF drugs are the most commonly used treatment for CNV. The biggest drawback of anti-VEGF drugs is the recurrence of CNV, which requires repeated therapy several times. Autophagy activation may be involved in reducing the therapeutic effect of anti-VEGF drugs. So, this study aims to elucidate the effect and mechanism of anti-VEGF drugs on endothelial autophagy and neovascularization in vitro. Methods RF/6A cells were randomly divided into five groups: The control group, hypoxia group (1% O2, 5% CO2, 94% N2), anti-VEGF group (group1: Ranibizumab 100 μg/ml; group2: Aflibercept, 400 μg/ml; group3: Conbercept, 100 μg/ml). Autophagy-related proteins were examined by Western blot. RFP-GFP-LC3 was used to detect autophagy and autophagic flow. Subsequently, we used autophagy inhibitors (3-MA or CQ) to inhibit Conbercept induced autophagy and to observe its effect on angiogenesis in vitro. Proliferation, migration, and tube formation of endothelial cells can be used to study neovascularization in vitro. In this research, the CCK-8 assay was used to detect cell proliferation. Cell migration and tube formation were assessed by wound assay and matrix method, respectively. Flow cytometry and Tunel were used to detect cell apoptosis. Finally, the mechanism of Conbercept activated autophagy was studied. Western blot was used to detect the expression of p53 and DRAM (damage-regulated autophagy modulator), upstream activators of autophagy. Results The protein levels of Beclin-1 and LC3-2/1 in Ranibizumab and Conbercept groups were significantly higher than in the hypoxia group(P  Conclusion Ranibizumab and Conbercept can trigger the autophagy of vascular endothelial cells while Aflibercept can inhibit it. The combination of Conbercept and autophagy inhibitor can significantly inhibit the formation of angiogenesis in vitro. The mechanism of autophagy activation is related to the activation of the p53/DRAM pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
水豚噜噜本人完成签到,获得积分10
23秒前
SciGPT的应助被怎样采纳,获得10
34秒前
MA_JT的应助被goh采纳,获得30
34秒前
东八圭完成签到,获得积分10
37秒前
风趣的雨莲完成签到,获得积分10
38秒前
月月发布了新的文献求助10
42秒前
LOONGLONG完成签到,获得积分10
46秒前
王震完成签到 ,获得积分10
52秒前
汉堡包的应助被月月采纳,获得10
55秒前
清爽的千易完成签到,获得积分10
1分钟前
李爱国的应助被方俊驰采纳,获得10
1分钟前
四夕完成签到 ,获得积分10
1分钟前
爆米花的应助被annzhu2005采纳,获得10
1分钟前
1分钟前
方俊驰发布了新的文献求助10
1分钟前
clover完成签到,获得积分10
1分钟前
A2311发布了新的文献求助10
1分钟前
Owen的应助被研友_闾丘枫采纳,获得10
1分钟前
1分钟前
快乐夜阑完成签到,获得积分10
1分钟前
1分钟前
blackia的应助被lusm_112采纳,获得50
1分钟前
星辰大海的应助被方俊驰采纳,获得10
1分钟前
科研通AI6.2的应助被李洪发采纳,获得10
1分钟前
2分钟前
方俊驰发布了新的文献求助10
2分钟前
2分钟前
whatadog发布了新的文献求助10
2分钟前
Hyp完成签到 ,获得积分10
2分钟前
呆萌的孤云完成签到,获得积分10
2分钟前
lusm_112发布了新的文献求助50
2分钟前
我是老大的应助被whatadog采纳,获得10
2分钟前
2分钟前
朴实的懿轩完成签到,获得积分10
2分钟前
2分钟前
33发布了新的文献求助10
2分钟前
聆月语关注了科研通微信公众号
2分钟前
白雪完成签到,获得积分10
2分钟前
2分钟前
Kiki发布了新的文献求助10
2分钟前
高分求助中
(应助此贴封号)通过应助OA文献获取积分 10000
Rosenblum, Global Change Biology 800
Computational Chemical Reaction Engineering: Modeling, Simulation, and Design with MATLAB 600
Organizational Behavior 510
Management and the Arts 510
Deformation and Fracture of the Lumbar Vertebral End Plate 500
CLSI C56QG Examples of Hemolyzed, Icteric, and Lipemic/Turbid Samples Quick Guide 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 计算机科学 工程类 纳米技术 内科学 物理 有机化学 化学工程 生物化学 复合材料 光电子学 细胞生物学 心理学 量子力学 催化作用 物理化学 电极
热门帖子
关注 科研通微信公众号,转发送积分 7802242
求助须知:如何正确求助?哪些是违规求助? 9336501
关于积分的说明 20479995
捐赠科研通 7393794
什么是DOI,文献DOI怎么找? 3326832
关于科研通互助平台的介绍 2473780
邀请新用户注册赠送积分活动 2344883