Berberine, a potential prebiotic to indirectly promote Akkermansia growth through stimulating gut mucin secretion

阿克曼西亚 粘蛋白 某种肠道细菌 生物 益生元 生物化学 微生物学 肠道菌群 乳酸菌 发酵
作者
Chunlan Dong,Jiaqi Yu,Yanan Yang,Fang Zhang,Wenquan Su,Qin-hua Fan,Chongming Wu,Shengxian Wu
出处
期刊:Biomedicine & Pharmacotherapy [Elsevier]
卷期号:139: 111595-111595 被引量:33
标识
DOI:10.1016/j.biopha.2021.111595
摘要

Akkermansia spp. plays important roles in maintenance of host health. Increasing evidence reveals that berberine (BBR) may exert its pharmacological effects via, at least partially, promotion of Akkermansia spp. However, how BBR stimulates Akkermansia remains largely unknown.In this study, we investigated the mechanism underlying the Akkermansia-promoting effect of BBR.The effect of BBR on Akkermansia was assessed in BBR-gavaged mice and direct incubation. The influence of BBR on intestinal mucin production was determined by alcian-blue staining and real-time PCR. The feces were analysis by gas chromatography-time-of-flight mass spectrometry (GC-TOF/MS) metabolomics. The role of polyamines in BBR-elicited mucin secretion and Akkermansia growth was evaluated by administration of difluoromethylornithine (DFMO) in mice.Gavage of BBR dose-dependently and time-dependently increased the abundance of Akkermansia in mice. However, it did not stimulate Akkermansia growth in direct incubation, suggesting that BBR may promote Akkermansia in a host-dependent way. Oral administration of BBR significantly increased the transcription of mucin-producing genes and mucin secretion in colon. Untargeted metabolomics analysis showed that BBR increased polyamines production in feces which are known to stimulate goblet cell proliferation and differentiation, but treatment with eukaryotic polyamine synthase inhibitor DFMO did not abolish the stimulating effect of BBR on mucin secretion and Akkermansia growth, indicating that the gut bacteria-derived but not the host-derived polyamines may involve in the BBR-promoted Akkermansia growth.Our results reveal that BBR is a promising prebiotic for Akkermansia, and it promotes Akkermansia growth via stimulating mucin secretion in colon.
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