辣根过氧化物酶
纳米化学
检出限
化学
酶
过氧化物酶
纳米载体
线性范围
色谱法
免疫分析
酶动力学
催化作用
纳米
抗体
生物化学
材料科学
有机化学
生物
药物输送
活动站点
复合材料
免疫学
作者
Pengyue Sun,Yao Li,Jing Li,Yaodong Zhang
标识
DOI:10.1007/s00604-021-05065-9
摘要
Horseradish peroxidase (HRP) was highly loaded into large holes of nanometer-scale metal–organic frameworks (i.e., PCN-333(Al)) for signal amplification in enzyme-linked immunosorbent assay (ELISA). The enzyme-labeled antibody complex prepared using nanometer-scale PCN-333(Al) maintained a high catalytic efficiency. Its Vm and Kcat values with 3,3',5,5'-Tetramethylbenzidine (TMB)-H2O2 as substrates were 4.84 × 10−5 mM/s and 4.84 × 104 min−1, respectively. We demonstrated an HRP@PCN-333 signal amplification strategy for colorimetric assay of human prostate-specific antigen (PSA). The linear range of PSA detection by using this method was 15–165 pg/mL, and the limit of detection was 6 pg/mL (S/N = 3), indicating the potential application of this method in detecting disease markers under clinical conditions. The presented strategy exhibited the characteristics of significantly increased amount of labeled enzymes, improved stability and utilization of enzymes, simple preparation process of enzyme-labeled antibodies, and low cost.Graphical abstract
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