固碳
格式化
自养
甘氨酸
生物化学
脱硫弧菌
氨
氢化酶
代谢途径
化学
细菌
新陈代谢
生物
氨基酸
酶
光合作用
遗传学
催化作用
作者
Irene Sánchez‐Andrea,Iamê Alves Guedes,Bastian Hornung,Sjef Boeren,Christopher E. Lawson,Diana Z. Sousa,Arren Bar‐Even,Nico J. Claassens,Alfons Johannes Maria Stams
标识
DOI:10.1038/s41467-020-18906-7
摘要
Abstract Six CO 2 fixation pathways are known to operate in photoautotrophic and chemoautotrophic microorganisms. Here, we describe chemolithoautotrophic growth of the sulphate-reducing bacterium Desulfovibrio desulfuricans (strain G11) with hydrogen and sulphate as energy substrates. Genomic, transcriptomic, proteomic and metabolomic analyses reveal that D. desulfuricans assimilates CO 2 via the reductive glycine pathway, a seventh CO 2 fixation pathway. In this pathway, CO 2 is first reduced to formate, which is reduced and condensed with a second CO 2 to generate glycine. Glycine is further reduced in D. desulfuricans by glycine reductase to acetyl-P, and then to acetyl-CoA, which is condensed with another CO 2 to form pyruvate. Ammonia is involved in the operation of the pathway, which is reflected in the dependence of the autotrophic growth rate on the ammonia concentration. Our study demonstrates microbial autotrophic growth fully supported by this highly ATP-efficient CO 2 fixation pathway.
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