胞嘧啶
基础(拓扑)
化学
对偶(语法数字)
生物化学
DNA
哲学
数学
数学分析
语言学
作者
Xiaohui Zhang,Biyun Zhu,Liang Chen,Ling Xie,Weishi Yu,Ying Wang,Linxi Li,Shuming Yin,Lei Yang,Handan Hu,Honghui Han,Yongmei Li,Liren Wang,Geng Chen,Xueyun Ma,Hongquan Geng,Wanfeng Huang,Xiufeng Pang,Zuozhen Yang,Yuxuan Wu
标识
DOI:10.1038/s41587-020-0527-y
摘要
Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax's activity on adenines is slightly reduced, whereas activity on cytosines is higher and RNA off-target activity is substantially decreased.
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