催化作用
级联
组合化学
对映选择合成
氢
胺气处理
产量(工程)
酒
氢键
酶
立体化学
作者
Francesco G. Mutti,Tanja Knaus,Nigel S. Scrutton,Michael Breuer,Nicholas J. Turner
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2015-09-25
卷期号:349 (6255): 1525-1529
被引量:242
标识
DOI:10.1126/science.aac9283
摘要
α-Chiral amines are key intermediates for the synthesis of a plethora of chemical compounds at industrial scale. We present a biocatalytic hydrogen-borrowing amination of primary and secondary alcohols that allows for the efficient and environmentally benign production of enantiopure amines. The method relies on a combination of two enzymes: an alcohol dehydrogenase (from Aromatoleum sp., Lactobacillus sp., or Bacillus sp.) operating in tandem with an amine dehydrogenase (engineered from Bacillus sp.) to aminate a structurally diverse range of aromatic and aliphatic alcohols, yielding up to 96% conversion and 99% enantiomeric excess. Primary alcohols were aminated with high conversion (up to 99%). This redox self-sufficient cascade possesses high atom efficiency, sourcing nitrogen from ammonium and generating water as the sole by-product.
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