单核苷酸多态性
小鼠苗条素受体
基因型
SNP公司
瘦素
内科学
医学
骨关节炎
限制性片段长度多态性
等位基因
等位基因频率
人口
多态性(计算机科学)
病例对照研究
内分泌学
遗传学
生物
基因
病理
肥胖
替代医学
环境卫生
作者
Xiaohong Ma,Hua Guo,Shao-Wen Hao,Shouxuan Sun,Xiaochun Yang,Bo Yu,Qunhua Jin
出处
期刊:Yichuan
[China Science Publishing & Media Ltd.]
日期:2013-09-27
卷期号:35 (3): 359-364
被引量:12
标识
DOI:10.3724/sp.j.1005.2013.00359
摘要
To investigate the association between primary knee osteoarthritis (OA) and single nucleotide polymorphism (SNP) (A668G) of leptin receptor gene (LEPR) in the Ningxia Hui population. A case-control association study has been adopted in this thesis. The polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis were performed to investigate the SNP of A668G site within LEPR from 148 patients with knee OA and 155 controls (asymptomatic and radiographically negative) with matched age and gender among Ningxia Hui population. In addition, genotypes of LEPR were verified by direct sequence analysis on PCR products. The result indicates that allele and genotype frequencies (P=0.024 and 0.008, respectively) in LEPR SNP A668G were significantly different in the knee OA patients group and control group, and in the knee OA patients group, the serum levels of leptin decreased significantly (P<0.001) and the serum levels of soluble leptin receptor increased significantly (P<0.001) compared with control group. Therefore, LEPR SNP A668G is associated with susceptibility to knee OA, which would be used as the genetic marker in predicting the risk of knee OA and would be one of the candidate genes in early prevention and control.
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