Influence of five pesticides (Carbendazim, Chlorothalonil and their mixture, Imidacloprid, Cypermethrin) on invertase activity in plastic shed soil in Changchun suburb was determined. Meanwhile, the invertase activity in the soils of plastic shed and farmland under developing pesticide function was compared and studied. Invertase activity in soil was inhibited markedly by Chlorothalonil, Cypermethrin and the mixture of Carbendazim-Chlorothalonil in the range of experiment concentration (0.1~50mg/g). At the concentration of Carbendazim and Imidacloprid below 0.1mg/g the invertase activity was stimulated, and inhibited at the concentration higher than 0.5mg/g. The joint use of Carbendazim and Chlorothalonil could strengthen obviously the toxicity of pesticide. The influence of different pesticide on the invertase activity in of plastic shed and farmland soils was different.