Isolation and Identification of WRKY Gene Family from Sweet Potato

The degenerate primers designed from the conserved motifs in the WRKY gene family were used to amplify WRKY gene family from Ipomoea batatas,Xushu18.15 WRKY gene family were obtained according to cloning and sequencing,and the amino acid sequence contained WRKYGQ and TTYEGKH(T / N / S / G / A / D)(H / Q) motifs of the WRKY.Cluster analysis of the predicted amino acid sequences grouped the WRKY of Ipomoea batatas and the known WRKY in NCBI database into five classes,and the sequence similarity was high.It was deduced that the RGAs with high similarity belonged to one gene family.