The Role of Mitochondria Mediated Apoptosis Pathway in Sertoli-germ Cell Apoptosis Induced by Manganese in Cocks

The aim of this study was to discuss the role of mitochondria mediated apoptosis pathway in Sertoli-germ cell apoptosis induced by manganese in cocks.Cock Sertoli-germ cells were cultivated in the DMEM for 24 h added with MnCl2,whose final concentrations were 0,2,3,and 4 mmol·L-1 respectively.The apoptosis was detected by AO/EB double staining.Mitochondrial transmembrane potential was detected by flow cytometer.Expression of Cytochrome c(Cytc)in cytolymph was detected by western blotting.The activities of Caspase-9,3 were examined by spectrophotography.Compared with control group,the apoptosis index(AI)of cock Sertoli-germ cells was significantly increased(P0.01),mitochondrial transmembrane potential was significantly decreased(P0.01),expression of Cytc in cytolymph was increased,and the activity of Caspase-9,3 were increased(P0.01)in 2,3,and 4 mmol·L-1 MnCl2 group.The apoptosis mediated by mitochondria pathway was one of the reproductive toxic mechanisms caused by manganese.