Treadmill test was performed on the source of four strains of Cordyceps militaris,and under the same of culture conditions,their growth was observed and compared;Cordycepin was obtained by ultrasonic extraction from different strains of mycelia with the same age.The results showed that: the peak area and the cordycepin content showed good linearity and high precision.The content of the Cordyceps mycelium YJ11-3 is the highest,0.505%,followed by YJ11-1 and YJ11-4,and YJ11-2 is the lowest,only 0.097%;Wet weight of mycelia is ordered as YJ11-3YJ11-1YJ11-4YJ11-2;Wet weight and content of the Cordyceps mycelium is proportional to each other.