Recombinase-Aid Amplification:a Novel Technology of in vitro Rapid Nucleic Acid Amplification

Nucleic acid amplification is a biotechnology that trace nucleic acid can be rapidly amplified in vitro.The technology has been applied widely in molecular biology,medical and forensic test etc.Such methodology has been continuously improved in its functionality and wideness of ultilization.Recombinase-aid amplification(RAA) is the most recent isothermal amplification technology,in which the classical thermal stable enzyme has been replaced by recombinase,single-stranded DNA binding(SSB) and DNA polymerase.Therefore,the RAA reaction can be carried out at optimized 37℃ or under the room temperatures without any heating assistance.The entire RAA reaction is very rapid,specific,power-saving and portable.These merits could largely facilitate RAA more applicable than PCR in the near future.