The sensitivity of soybean Dongnong 50 to herbicide glufosinate was analyzed.The results showed that leaves became withered and yellow after treated with 50 mg·L-1 glufosinate for three days.The effect of herbicide phytotoxicity increased with concentration increase.The sensitivity of soybean plants to glufosmate was different,new leaves and cotyledon were more sensitive to glufosinate than stem.Spraying with 75 mg · L-1 glufosinate over the whole plant showed significant phytotoxicity,which was easy to be observed with duplicate spray.