定量分析(化学)
计算机科学
生化工程
质谱法
定量评估
计算生物学
纳米技术
化学
色谱法
生物
风险分析(工程)
材料科学
医学
工程类
作者
Xiaobo Yin,Zhichao Xue,Lulu Feng,Siyuan Tan,Kangming Li,Jing You,Xiaoyun Gong,Xinhua Dai,Xiang Fang
标识
DOI:10.1016/j.trac.2023.117503
摘要
Quantitative analysis of bio-molecules in single cells offers a direct and precise way for us to understand cell heterogeneity more comprehensively. Mass spectrometry (MS) based methods hold the exclusive advantages of unparalleled sensitivity and accuracy in the application of single-cell quantitative analysis. Excellent works have been done in recent years. This review summarizes the most recent advances in the development and applications of novel MS based quantitative methods. Sampling, pre-treatment, and ionization techniques that improve the sensitivity and accuracy of the methods are dissected comprehensively. The advantages, limitations and application scopes of different methods are compared and discussed in detail. This review offers us insights into the development trends in single-cell analysis from a quantitative perspective. It is beneficial to the fundamental researches in cell biology and medicine. It also provides guidance to the selection of suitable quantitative methods in biological and clinical applications.
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