JNK-ERK Synergistic Regulation of P450 Gene Expression Confers Nitenpyram Resistance in Nilaparvata lugens (Stål)

Cascading regulation of signaling pathways plays a crucial role in insect growth, development, and adaptation. However, how insects employ signaling cascades to regulate detoxification gene expression and enhance resistance is not well understood. In the current study, we investigated the MAPK signaling pathway in mediating nitenpyram resistance in Nilaparvata lugens. qRT-PCR and western-blot analyses revealed that both transcription and protein levels of NlJNK and NlERK were upregulated in the nitenpyram resistant strain, and these changes can be induced by exposure to nitenpyram. Moreover, the expression of P450 genes including NlCYP6ER1, NlCYP302A1, and NlCYP6AY1, which were closely associated with nitenpyram resistance, was significantly decreased following the silencing of NlJNK and NlERK or inhibitor treatments. Further studies showed that NlERK–NlJNK comediated transcription factors NlCREB and NlAP-1 to regulate P450 gene expression. These findings highlight the critical role of the MAPK pathway in N. lugens resistance and offer the potential targets for the insecticide resistance management.