Streptomyces haneummycinicus sp. nov., a new antibiotic-producing actinomycete isolated from marine sediment

An actinomycete strain that produces a new antibiotic haneummycin, designated KM77-8 T , was isolated from marine sediment collected from Tokyo Bay near Haneda Airport, Japan. This strain was phylogenetically related to Streptomyces pilosus NBRC 12807 T , with the highest similarity (99.65%) based on 16S rRNA gene sequences. In contrast, genome-based comparisons between strain KM77-8 T and S. pilosus JCM 4403 T (=NBRC 12807 T ) revealed that average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values were 87.29% and 33.00 %, respectively. Moreover, genome-based comparisons between strain KM77-8 T and other known Streptomyces species, including close relatives (more than 98.7% similarities of 16S rRNA gene sequences), showed that their ANI and dDDH values were <90% and <70%, respectively. The DNA G+C content of strain KM77-8 T was 71.34%. The whole-cell hydrolysates of strain KM77-8 T contained ll -diaminopimelic acid, galactose, glucose, mannose and ribose. The major menaquinones (MKs) of this strain were MK-9(H 6 ) and MK-9(H 8 ), and the major fatty acids (>10%) were anteiso-C 15 : 0 , iso-C 15 : 0 , C 16 : 0 , iso-C 16 : 0 and summed feature 3 (C 16 : 1 ω7 c / C 16 : 1 ω6 c). Several phenotypic differences were observed between strain KM77-8 T and S. pilosus NBRC 12807 T . Furthermore, supernatant and methanol extract of not S. pilosus NBRC 12807 T but strain KM77-8 T displayed antibacterial activity against Enterococcus faecium and contained haneummycin. On the basis of the polyphasic data, we propose strain KM77-8 T as a novel species of the genus Streptomyces , named Streptomyces haneummycinicus sp. nov. The type strain is KM77-8 T (=NBRC 115995 T =DSM 115857 T ).