舍瓦内拉
死水
微生物燃料电池
制浆造纸工业
化学
废物管理
食品科学
细菌
生物
工程类
电极
遗传学
阳极
发酵
物理化学
作者
Junqi Zhang,Dan Wu,Yakun Zhao,Dingyuan Liu,Xuewu Guo,Yefu Chen,Cuiying Zhang,Xi Sun,Jin Guo,Dezhi Yuan,Dongguang Xiao,Feng Li,Hao Song
标识
DOI:10.1016/j.scitotenv.2022.158696
摘要
Thin stillage, rich in glucose and lactate, can seriously pollute water resources when directly discharged into the natural environment. Microbial fuel cells (MFC), as a green and sustainable technology, could utilize exoelectrogens to break down organics in wastewater and harvest electricity. Nevertheless, Shewanella oneidensis MR-1, cannot utilize thin stillage for efficient power generation. Here, to enable S. oneidensis to co-utilize glucose and lactate from thin stillage, an engineered S. oneidensis G7∆RSL1 was first created by constructing glucose metabolism pathway, promoting glucose and lactate co-utilization, and enhancing biofilm formation. Then, to enhance biofilm conductivity, we constructed a 3D self-assembled G7∆RSL1-rGO/CNT biohybrid with maximum power density of 560.4 mW m-2 and 373.7 mW m-2 in artificial and actual thin stillage, respectively, the highest among the reported genetically engineered S. oneidensis with thin stillage as carbon source. This study provides a new strategy to facilitate practical applications of MFC in wastewater remediation and efficient power recovery.
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