DNA‐Based Artificial Receptors as Transmembrane Signal Transduction Systems for Protocellular Communication

Abstract The ability to reproduce signal transduction and cellular communication in artificial cell systems is significant in synthetic protobiology. Here, we describe an artificial transmembrane signal transduction through low pH‐mediated formation of the i‐motif and dimerization of DNA‐based artificial membrane receptors, which is coupled to the occurrence of fluorescence resonance energy transfer and the activation of G‐quadruplex/hemin‐mediated fluorescence amplification inside giant unilamellar vesicles. Moreover, an intercellular signal communication model is established when the extravesicular H + input is replaced by coacervate microdroplets, which activate the dimerization of the artificial receptors, and subsequent fluorescence production or polymerization in giant unilamellar vesicles. This study represents a crucial step towards designing artificial signalling systems with environmental response, and provides an opportunity to establish signalling networks in protocell colonies.