荧光
检出限
适体
猝灭(荧光)
胶体金
化学
生物传感器
离子
沙门氏菌
纳米技术
共价键
材料科学
纳米颗粒
光化学
色谱法
细菌
有机化学
分子生物学
物理
量子力学
生物
遗传学
作者
Shengnan Wei,Feng Wang,Liang Zhang,Chao Zhao,Juan Li,Juan Wang
标识
DOI:10.1016/j.snb.2023.134076
摘要
The core/shell magnetic covalent organic framework doped with gold nanoparticles (MCOF-AuNPs) was prepared by a facile two-step in situ synthesis strategy to achieve high emission. The fluorescence emission was enhanced and overcame the aggregation-induced quenching of COF after the introduction of Fe3O4 and AuNPs. Interestingly, the emission can be quenched by Fe3+ ions. MCOF-AuNPs combined with a specific aptamer (apt-MCOF-AuNPs) can capture Salmonella typhimurium selectively, and Fe3+-induced fluorescence quenching was inhibited based on the competitive binding of Fe3+ ions. A fluorescent assay strategy was constructed with excellent specificity and sensitivity. The detection limit is as low as 4 CFU/mL within 33 min. Furthermore, we developed smartphone-based software as an image reader and analyzer to determine S. typhimurium by capturing the change in fluorescence lightness, which can be assisted for point-of-care testing. Collectively, this innovative design showed a promising on-site detection approach for S. typhimurium.
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