Target-induced recycling and self-folding hairpin primer-mediated LAMP activation of CRISPR/Cas12a for highly sensitive aptamer-based therapeutic antibody assay

-cleavage property for cyclically cutting ssDNA reporter molecules and yielding considerably magnified fluorescence recovery for trastuzumab detection. Owing to target-recycling, LAMP and Cas12a/crRNA-integrated signal amplifications, a low picomolar detection limit (4.17 pM) for trastuzumab was achieved. This assay could also be applied to trace trastuzumab in diluted human serums. With the distinct advantages of low-temperature LAMP activation with minimal primer involvement and the integration of an amplification cascade, this sensing methodology could be employed as a robust signal enhancement methodology for detecting various molecular biomarkers for diverse biomedical and biological applications.