FeMoO 4 nanospheres-based nanozymatic colorimetry for rapid and sensitive pyrophosphate detection

catalysis. Due to the Fe(II) and PPi reaction, the presence of PPi could specifically restore blue oxidized TMB to colorless TMB, which led to a decrease in UV absorption at 652 nm. The absorbance change is proportional to the PPi concentration, with a linear detection range (from 0.5 to 25 μM) and a low detection limit of 0.3 μM (S/N = 3). Accordingly, its excellent selectivity and high sensitivity made it a potential colorimetric sensor for PPi analysis in actual water samples.