胰腺
传出的
生物
转基因小鼠
胆碱能的
转基因
神经科学
逆行追踪
内分泌学
内科学
中枢神经系统
医学
传入的
基因
生物化学
作者
Maria Jiménez-González,R. Li,Lisa E. Pomeranz,Alexandra Alvarsson,Roberta Marongiu,Rollie F. Hampton,Michael G. Kaplitt,R. C. Vasavada,Gary J. Schwartz,Sarah A. Stanley
标识
DOI:10.1038/s41551-022-00909-y
摘要
A lack of comprehensive mapping of ganglionic inputs into the pancreas and of technology for the modulation of the activity of specific pancreatic nerves has hindered the study of how they regulate metabolic processes. Here we show that the pancreas-innervating neurons in sympathetic, parasympathetic and sensory ganglia can be mapped in detail by using tissue clearing and retrograde tracing (the tracing of neural connections from the synapse to the cell body), and that genetic payloads can be delivered via intrapancreatic injection to target sites in efferent pancreatic nerves in live mice through optimized adeno-associated viruses and neural-tissue-specific promoters. We also show that, in male mice, the targeted activation of parasympathetic cholinergic intrapancreatic ganglia and neurons doubled plasma-insulin levels and improved glucose tolerance, and that tolerance was impaired by stimulating pancreas-projecting sympathetic neurons. The ability to map the peripheral ganglia innervating the pancreas and to deliver transgenes to specific pancreas-projecting neurons will facilitate the examination of ganglionic inputs and the study of the roles of pancreatic efferent innervation in glucose metabolism.
科研通智能强力驱动
Strongly Powered by AbleSci AI