化学
过氧化氢酶
多巴胺
纳米技术
色谱法
生物化学
酶
神经科学
生物
材料科学
作者
Anqi Lin,Quanyi Liu,Yihong Zhang,Quan Wang,Sirong Li,Bijun Zhu,Leiying Miao,Yan Du,Sheng Zhao,Hui Wei
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2022-06-27
卷期号:94 (30): 10636-10642
被引量:21
标识
DOI:10.1021/acs.analchem.2c00804
摘要
Developing a universal strategy to measure catalase (CAT)/CAT-like activity, on one hand, overcomes limitations on current assays, such as moderate sensitivity and limited sample scope; on the other hand, facilitates insightful studies on applications of CAT and CAT-like nanozymes. Herein, the oxygen-sensitive and H2O2-inhibitory self-polymerization of dopamine (DA) was demonstrated as an activity indicator of CAT or CAT-like nanozymes, which monitors the catalytically generated O2 in a hypoxic environment. A typical assay for natural CAT was achieved under the optimized conditions. Moreover, this assay was suitable for diverse types of samples, ranging from nanozymes, animal tissues, to human saliva. By comparing the merits and limitations of common methods, this assay shows all-round advantages in sensitivity, specificity, and versatility, facilitating the formulation of measurement criteria and the development of potential standardized assays for CAT (or CAT-like nanozyme) activity.
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