Cellulase Assays

Various kinetic models have been developed to determine the effectiveness of cellulase enzyme preparations on the rate and extent of cellulose hydrolysis. The assay method proposed by Sattler et al. ( 1 ) was used to compare five dilute acid treated wood sawdust samples and a control microcrystalline cellulose, Sigmacell 50. This study confirmed that where kinetic information is required for a specific biomass conversion process application, such as simultaneous saccharification and fermentation (SSF), hydrolysis kinetics must be evaluated using the actual substrate to acquire meaningful results.