谷胱甘肽S-转移酶
谷胱甘肽
蛋白质组学
定量蛋白质组学
代谢组学
化学
胰蛋白酶
计算生物学
质谱法
色谱法
生物化学
生物
酶
基因
作者
Yuxing Zhang,Deliang Huang,Ning Lv,Guilan Zhu,Jinghan Peng,Tiansheng Chou,Zhibin Zhu,Ju Wang,Yuanyuan Chen,Xiangdong Fang,Jiuxin Qu,Jun Chen,Siqi Liu
标识
DOI:10.1021/acs.jproteome.2c00049
摘要
The members of the glutathione S-transferase (GST) superfamily often exhibit functional overlap and can compensate for each other. Their concentrations in serum are considered as disease biomarkers. A global and quantitative evaluation of serum GSTs is therefore urgent, but there is a lack of efficient approaches due to technological limitations. GSH magnetic beads were examined for their affinity to enrich GSTs in serum, and the enriched GSTs were quantitatively targeted using a Q Exactive HF-X mass spectrometer in parallel reaction monitoring (PRM) mode. To optimize the quantification of GST peptides, sample types, trypsin digestion, and serum loading were carefully assessed; a biosynthetic method was employed to generate isotope-labeled GST peptides, and instrumental parameters were systematically optimized. A total of 134 clinical sera were collected for GST quantification from healthy donors and patients with four liver diseases. Using the new approach, GSTs in healthy sera were profiled: 14 GST peptides were quantified, and the abundance of five GST families was ranked GSTM > GSTP > GSTA > MGST1 > GSTT1, ranging from 0.1 to 4 pmol/L. Furthermore, combining the abundance of multiple GST peptides could effectively distinguish different types of liver diseases. Quantification of serum GSTs through targeted proteomics, therefore, has apparent clinical potential for disease diagnosis.
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