| 标题 |
 Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli 相关领域
大肠杆菌
异源的
密码子使用偏好性
异源表达
蛋白质表达
生物
计算生物学
重组DNA
遗传学
基因
基因组
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| DOI | |
| 其它 | Protein expression is a critical process in diverse biological systems. For Escherichia coli, a widely employed microbial host in industrial catalysis and healthcare, researchers often face significant challenges in constructing recombinant expression systems. To maximize the potential of E. coli expression systems, it is essential to address problems regarding the low or absent production of certain target proteins. This article presents viable solutions to the main factors posing challenges to heterologous protein expression in E. coli, which includes protein toxicity, the intrinsic influence of gene sequences, and mRNA structure. These strategies include specialized approaches for managing toxic protein expression, addressing issues related to mRNA structure and codon bias, advanced codon optimization methodologies that consider multiple factors, and emerging optimization techniques facilitated by big data and machine learning. |
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