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Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29 相关领域
解淀粉芽孢杆菌
脂肪酶
淀粉酶
食品科学
酶
蛋白酶
芽孢杆菌(形态)
化学
酯交换
生物化学
生物
微生物学
发酵
催化作用
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A strain of Bacillus amyloliquefaciens KUB29 was identified by 16S ribosomal RNA sequencing (Genbank: MF772779.1). Production of thermostable protease, amylase and lipase were done by the isolated strain. The produced enzymes were partially purified by ammonium precipitation followed by dialysis process. Protease and lipase enzymes are effectively used in bio-oil extraction from proteinaceous sample followed by transesterification to produce methyl ester. Amylase enzyme is widely used in food and laundry industry. The produced enzymes are active at thermophilic condition of 55 °C. Use of these enzymes in biofuel production process will make the process cleaner and greener. 通过16S核糖体RNA测序(Genbank:MF77279.1)鉴定了一株解淀粉芽孢杆菌KUB29。分离菌株生产耐热蛋白酶、淀粉酶和脂肪酶。通过铵沉淀和透析过程对产生的酶进行部分纯化。蛋白酶和脂肪酶有效地用于从蛋白质样品中提取生物油,然后通过酯交换反应生产甲酯。淀粉酶广泛应用于食品和洗衣行业。所产生的酶在55°C的高温条件下具有活性。在生物燃料生产过程中使用这些酶将使该过程更清洁、更环保。 |
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(2025-6-4)