| 标题 |
Effect of dimethyl sulfoxide on in vitro proliferation of skin fibroblast cells |
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| 其它 |
Mahipal Singh; Kya McKenzie; Xiaoling Ma Abstract / 摘要MT翻译 Dimethyl sulfoxide is a preferred solvent for a wide range of compounds. It is used as a solvent of drugs for in-vitro toxicity testing using cell lines. Determination of safe solvent concentration for cell type to be used is a pre-requirement. Here we show that 0.1% DMSO in growth media is the safest concentration for skin fibroblast cell line used in this study. The low concentrations (0.01-0.001%) enhance proliferation of cells when cultured in-vitro on plastic surfaces for a 4-day period, whereas higher concentrations (0.5-3%) lead to reduction in cell-viability on a dose dependent manner. No cell survived beyond 3% DMSO. |
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(2025-6-4)