Targeted next generation sequencing is comparable with metagenomic next generation sequencing in adults with pneumonia for pathogenic microorganism detection

We have read with great interest the study published in this journal by Peng et al.,1 comparing the potential of metagenomic next-generation sequencing (mNGS) with that of comprehensive conventional microbiological tests (CMTs) as a front-line diagnostic in immunocompromised patients with suspected pneumonia. mNGS has markedly improved the efficiency of pneumonia's etiological diagnosis. Pathogens can be identified by mNGS include bacteria,2 RNA and DNA viruses,3 yeast and molds,4 mycobacteria and parasites.