Multiple signal amplification strategy for ultrasensitive sensing of Mycobacterium bovis based on 8–17 DNAzyme and CRISPR-Cas13a

In this study, we developed a triple-cycle amplification system that connected the 8–17 DNAzyme cleavage, CRISPR-Cas13a-mediated cleavage and CHA in series, demonstrating high sensitivity and specificity in detecting Mycobacterium bovis .