血红素
溶血
细胞外
钙
化学
生物物理学
生物化学
血红素
生物
免疫学
酶
有机化学
作者
Diana M. Mikhailova,Elisaveta Skverchinskaya,Julia Sudnitsyna,Kirill R. Butov,E. M. Koltsova,Igor Mindukshev,Stepan Gambaryan
出处
期刊:Cells
[MDPI AG]
日期:2024-03-21
卷期号:13 (6): 554-554
标识
DOI:10.3390/cells13060554
摘要
Pathologies such as malaria, hemorrhagic stroke, sickle cell disease, and thalassemia are characterized by the release of hemoglobin degradation products from damaged RBCs. Hematin (liganded with OH−) and hemin (liganded with Cl−)—are the oxidized forms of heme with toxic properties due to their hydrophobicity and the presence of redox-active Fe3. In the present study, using the original LaSca-TM laser particle analyzer, flow cytometry, and confocal microscopy, we showed that both hematin and hemin induce dose-dependent RBC spherization and hemolysis with ghost formation. Hematin and hemin at nanomolar concentrations increased [Ca2+]i in RBC; however, spherization and hemolysis occurred in the presence and absence of calcium, indicating that both processes are independent of [Ca2+]i. Both compounds triggered acute phosphatidylserine exposure on the membrane surface, reversible after 60 min of incubation. A comparison of hematin and hemin effects on RBCs revealed that hematin is a more reactive toxic metabolite than hemin towards human RBCs. The toxic effects of heme derivatives were reduced and even reversed in the presence of albumin, indicating the presence in RBCs of the own recovery system against the toxic effects of heme derivatives.
科研通智能强力驱动
Strongly Powered by AbleSci AI