阿达尔
RNA解旋酶A
基因敲除
生物
核糖核酸
三素数非翻译区
RNA编辑
翻译(生物学)
非翻译区
核酸结构
细胞生物学
分子生物学
信使核糖核酸
解旋酶
遗传学
基因
作者
Kaixin Lyu,Shuo-Bin Chen,Elaine Chow,Haizhou Zhao,Jiahao Yuan,Meng Cai,Jiahai Shi,Ting‐Fung Chan,Jia‐Heng Tan,C. Y. Kwok
标识
DOI:10.1002/anie.202203553
摘要
Abstract RNA G‐quadruplex (rG4) structures in the 5′ untranslated region (5′UTR) play crucial roles in fundamental cellular processes. ADAR is an important enzyme that binds to double‐strand RNA and accounts for the conversion of Adenosine to Inosine in RNA editing. However, so far there is no report on the formation and regulatory role of rG4 on ADAR expression. Here, we identify and characterize a thermostable rG4 structure within the 5′UTR of the ADAR1 mRNA and demonstrate its formation and inhibitory role on translation in reporter gene and native gene constructs. We reveal rG4‐specific helicase DHX36 interacts with this rG4 in vitro and in cells under knockdown and knockout conditions by GTFH (G‐quadruplex‐triggered fluorogenic hybridization) probes and modulates translation in an rG4‐dependent manner. Our results further substantiate the rG4 structure‐DHX36 protein interaction in cells and highlight rG4 to be a key player in controlling ADAR1 translation.
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