免疫球蛋白E
过敏原
免疫系统
食物过敏
生物传感器
食物过敏原
抗体
化学
纳米技术
过敏
生物
免疫学
生物化学
材料科学
作者
Jin-Young Jeong,So-ong Kim,Sunwoo Bang,Young-Hee Choi,Jaikwang Shin,Danmin Choi,Sang-Eun Lee,Tai Hyun Park,Seunghun Hong
标识
DOI:10.1016/j.bios.2022.114914
摘要
Inspired by an adaptive immune system, we have developed a bioelectronic sensing platform which relies on nanovesicles for a signal amplification and can be easily adapted for the detection of new food allergens. In this work, nanovesicles with anti-immunoglobulin E (anti-IgE) antibody receptors were extracted from immune cells and immobilized on a carbon nanotube-based transistor to build a highly sensitive and selective biosensing platform. Our sensor could detect peanut allergen, arachis hypogaea 2 (Ara h 2), down to 0.1 fM and selectively discriminate target allergens in real food samples such as peanut and egg white. As a proof of concept, we demonstrated the detection of different target molecules using the same nanovesicles linked with different antibodies. Our sensor platform was also utilized to quantitatively evaluate the effect of allergy drug such as cromolyn. In this regard, our strategy can be utilized for basic research and versatile applications in food and pharmacological industries.
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