非翻译区
信使核糖核酸
核糖核酸
三素数非翻译区
蛋白质生物合成
甘氨酸
转基因
细胞生物学
生物合成
生物
转录后调控
基因
植物
化学
生物化学
氨基酸
作者
Dian Wang,Xiaoning Hao,Li Xu,Mengyan Zhao,Congpeng Wang,Xihao Yu,Yingzhen Kong,Mengzhu Lu,Gongke Zhou,Guohua Chai,Xianfeng Tang
摘要
Brassinosteroids (BRs) are plant hormones that regulate wood formation in trees. Currently, little is known about the post-transcriptional regulation of BR synthesis. Here, we show that during wood formation, fine-tuning BR synthesis requires 3'UTR-dependent decay of Populus CONSTITUTIVE PHOTOMORPHOGENIC DWARF 1 (PdCPD1). Overexpression of PdCPD1 or its 3′ UTR fragment resulted in a significant increase of BR levels and inhibited secondary growth. In contrast, transgenic poplars repressing PdCPD1 3′ UTR expression displayed moderate levels of BR and promoted wood formation. We show that the Populus GLYCINE-RICH RNA-BINDING PROTEIN 1 (PdGRP1) directly binds to a GU-rich element in 3′ UTR of PdCPD1, leading to its mRNA decay. We thus provide a post-transcriptional mechanism underlying BRs synthesis during wood formation, which may be useful for genetic manipulation of wood biomass in trees.This article is protected by copyright. All rights reserved.
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