In this study the effect of incorporation of aqueous extract of Agave tequilana (AEAT) on viability of Bifidobacterium bifidum and Lactobacillus acidophilus was investigated. No reduction of cell viability was seen in any treatment groups of L. acidophilus during the storage time. A statistically significant increase in L. acidophilus count was found in synbiotic yogurt samples containing 1 and 1.5% concentrations of the extract at day 21 of storage compared to the first day (p 0.05). All the yogurt samples revealed an increase in titratable acidity as a function of storage time. In conclusion, as the results of this study indicated improvement of probiotic bacteria in yogurt incorporated with AEAT, its potential application as a functional food formulation is recommended.