化学
催化作用
生物传感器
纳米颗粒
检出限
荧光
基质(水族馆)
组合化学
纳米技术
过氧化物酶
材料科学
酶
色谱法
有机化学
生物化学
物理
地质学
海洋学
量子力学
作者
Chan Ho Song,Haibo Liu,Linlin Zhang,Jie Jin Wang,Chenglong Zhao,Qinyuan Xu,Cheng Yao
标识
DOI:10.1016/j.snb.2021.131131
摘要
Nanozymes with high catalytic performance play a vital role in the construction of advanced biosensors for the signal detection and analysis of complicated biological samples. Herein, FeS nanoparticles (NPs) embedded in 2D carbon nanosheets (denoted as [email protected]) are demonstrated to serve as excellent nanozymes with high catalytic activity. Notably, the introduction of mesoporous carbon substrate could effectively improve the enzymatic peroxidase-like catalytic ability of embedded FeS nanozymes, especially in the fluorescence catalytic system. Based on the high peroxidase-like activity of proposed [email protected] nanozymes, a facile method was then designed for H2O2 assay with detection limit as low as 0.78 μM and 0.86 μM for the colorimetric and fluorescence method, respectively. The oxidation of the colorimetric and fluorescence substrates could be ascribed to the hydroxyl radical (·OH) produced by catalytic reaction of H2O2 with [email protected] nanozymes. Thus, the [email protected] nanozymes-based H2O2 sensing platform was further constructed to evaluate the antioxidant capacity of glutathione (GSH), cysteine (Cys), and gallic acid (GA). Notably, [email protected] nanozymes can keep the functionality in a broad pH range (2.0 ~ 9.0) for catalytic reaction, delivering a great potential in the fabrication of advanced biosensors.
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