反式激活crRNA
清脆的
计算生物学
DNA
生物传感器
纳米技术
检测点注意事项
计算机科学
Cas9
生物信息学
生物
遗传学
基因
生物化学
材料科学
免疫学
作者
Yidan Tang,Lijuan Qi,Yichen Liu,Lulu Guo,Rujian Zhao,Meng Yang,Yan Du,Bingling Li
标识
DOI:10.1002/anie.202115907
摘要
Desirable biosensing assays need to be sensitive, specific, cost-effective, instrument-free, and versatile. Herein we report a new strategy termed CLIPON (CRISPR and Large DNA assembly Induced Pregnancy strips for signal-ON detection) that can deliver these traits. CLIPON integrates a commercial pregnancy test strip (PTS) with four biological elements: the human chorionic gonadotropin (hCG), CRISPR-Cas12a, crRNA and cauliflower-like large-sized DNA assemblies (CLD). CLIPON uses the Cas12a/crRNA complex both to recognize a target of interest and to release CLD-bound hCG so that target presence can translate into a colorimetric signal on the PTS. We demonstrate the versatility of CLIPON through sensitive and specific detection of HPV genomic DNA, SARS-CoV-2 genomic RNA and adenosine. We also engineer a cell phone app and a hand-held microchip to achieve signal quantification. CLIPON represents an attractive option for biosensing and point-of-care diagnostics.
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