The experiment was conducted to assess the effect of three typical Trichoderma spp. (T. Harzianum or T1, T. Asperellum or T2 and T. virens or T3) alone or in combination on pathogens of Fusarium solani and F. oxysporum as agents of root rot in Cucumber ( Cucumis sativus ) field. A total twenty experimental cucumber fields was considered for study on basis of a completely randomized block design (with 4 replicates, 9 plants per). Plants were planted in Fusarium pre-infested soil and then treated with one or mix of Trichoderma spp. (T1, T2, T3, T1+T2, T1+T3, T2+T3, T1+T2+T3) for detecting the best bio-control treatment against pathogens. The F. solani -infested fields treated with mix trio of Trichoderma spp. (T1+T2+T3), showed the lowest external yellowing and dropping , root discoloration and disease incidence (10.50%, 6.50% and 11.25%, respectively). Better bio-control of F. solani and F. oxysprom was also done by different combination of antagonist funguses (80-84%). It concluded that the combination of Trichoderma spp. showed the more protective effects on cucumber root rots agents (Fusarium ) and it can be considered as a protective strategy in fruiting plants for reduce the negative effects of infectious pathogens.