The regenerated Antheraea pernyi(A.pernyi) silk fibroin films were prepared from Lithium thiocyanate solution.Physico-chemical methods were used for the analysis of the structure characteristics and the change of molecular conformation when exposed in air.The structure of regenerated A.pernyi silk fibroin films was the coexistence of αhelix and random coil.After kept in air for 30 days,part of the random coil transformed to αhelix,but no βsheet structure formed.