Orientation and analysis of Yuyi hairless mice hairless gene mutation point

Aim:To research the hairless gene mutation of Yuyi hairless mice.Methods:Partial hairless gene of Yuyi hairless mice and Kunming mice were cloned,sequenced,and compared with Mus musculus DNA.Results:The DNA fragment of Yuyi hairless mice contained 1 824 bp,that of Kunming mice contained 1 816 bp.There were 27 differences,including insertion,deletion,and point mutation.One nonsense mutation in exon 12 resulted in the introduction of a premature termination codon UGA.Conclusion:Hairless character is probably associated with the hairless gene mutation of Yuyi hairless mice.