Effect of Astragaloside IV on the Proliferation and Cell Factor Expression in hBMSC

Objective: To investigate the effect of Astragaloside IV on the proliferation and the expression of cell factor in human bone marrow mesenchymal stem cells(hBMSC). Methods: Human bone marrow mesenchymal stem cells were isolated from bone marrow by percoll isodensity centrifugation and adherent method. The surface marker of hBMSC were detected by flow cytometer. The proliferation of hBMSC with different concentration Astragaloside IV for 72 hours were evaluated by MTT. The expressions of SCF, VEGF, SDF-1, GM-CSF mRNA were measured by Real-time PCR. Results: The hBMSC were isolated successfully. Astragaloside IV at 20, 40, 80, 160, 320 mg/mL significantly increased the proliferation of hBMSC(P0.05), especially the group of 160 mg/mL had the most obvious effect. Astragaloside IV could stimulated the expressions of SCF, VEGF, SDF-1 mRNA, but GM-CSF mRNA were not change significantly. Conclusions: Astragaloside IV could increase the proliferation of hBMSC and improve the expressions of the SCFV, EGF, SDF-1 mRNA.